Deoxyxylulose 5-Phosphate Synthase Controls Flux through the Methylerythritol 4-Phosphate Pathway in Arabidopsis1[C][W][OPEN]
نویسندگان
چکیده
Department of Biochemistry, Max Plank Institute for Chemical Ecology, 07745 Jena, Germany (L.P.W., A.H., B.R., J.G.); Department of Biochemistry, Stellenbosch University, 7602 Stellenbosch, South Africa (J.M.R.); Research Unit Environmental Simulation (EUS), Institute of Biochemical Plant Pathology, Helmholtz Zentrum, 85764 Neuherberg, Germany (A.G., J.-P.S.); and Plant Metabolism and Metabolic Engineering Program, Centre for Research in Agricultural Genomics (Consorci CSIC-IRTA-UAB-UB), 08193 Bellaterra, Barcelona, Spain (M.O., M.A.P.)
منابع مشابه
Deoxyxylulose 5-Phosphate Synthase Controls Flux through the Methylerythritol 4-Phosphate Pathway in Arabidopsis.
The 2-C-methylerythritol 4-phosphate (MEP) pathway supplies precursors for plastidial isoprenoid biosynthesis including carotenoids, redox cofactor side chains, and biogenic volatile organic compounds. We examined the first enzyme of this pathway, 1-deoxyxylulose 5-phosphate synthase (DXS), using metabolic control analysis. Multiple Arabidopsis (Arabidopsis thaliana) lines presenting a range of...
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2-C-methylerythritol 4-phosphate has been established recently as an intermediate of the deoxyxylulose phosphate pathway used for biosynthesis of terpenoids in plants and in many microorganisms. We show that an enzyme isolated from cell extract of Escherichia coli converts 2-C-methylerythritol 4-phosphate into 4-diphosphocytidyl-2-C-methylerythritol by reaction with CTP. The enzyme is specified...
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Isoprenoid biosynthesis via the methylerythritol phosphate pathway is a target against pathogenic bacteria and the malaria parasite Plasmodium falciparum. 4-(Hydroxyamino)-4-oxobutylphosphonic acid and 4-[hydroxy(methyl)amino]-4-oxobutyl phosphonic acid, two novel inhibitors of DXR (1-deoxy-D-xylulose 5-phosphate reducto-isomerase), the second enzyme of the pathway, have been synthesized and co...
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